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J Comp Neurol. 2017 Apr 1;525(5):1291-1311. doi: 10.1002/cne.24137. Epub 2016 Nov 21.

In vivo transgenic expression of collybistin in neurons of the rat cerebral cortex.

Author information

1
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.

Abstract

Collybistin (CB) is a guanine nucleotide exchange factor selectively localized to γ-aminobutyric acid (GABA)ergic and glycinergic postsynapses. Active CB interacts with gephyrin, inducing the submembranous clustering and the postsynaptic accumulation of gephyrin, which is a scaffold protein that recruits GABAA receptors (GABAA Rs) at the postsynapse. CB is expressed with or without a src homology 3 (SH3) domain. We have previously reported the effects on GABAergic synapses of the acute overexpression of CBSH3- or CBSH3+ in cultured hippocampal (HP) neurons. In the present communication, we are studying the effects on GABAergic synapses after chronic in vivo transgenic expression of CB2SH3- or CB2SH3+ in neurons of the adult rat cerebral cortex. The embryonic precursors of these cortical neurons were in utero electroporated with CBSH3- or CBSH3+ DNAs, migrated to the appropriate cortical layer, and became integrated in cortical circuits. The results show that: 1) the strength of inhibitory synapses in vivo can be enhanced by increasing the expression of CB in neurons; and 2) there are significant differences in the results between in vivo and in culture studies. J. Comp. Neurol. 525:1291-1311, 2017.

KEYWORDS:

GABAA receptors; GABAergic synapse; GABAergic synaptic plasticity; GABAergic synaptic strength; RRID: AB_10064220; RRID: AB_2232546; RRID: AB_2314482; RRID: AB_887873; RRID: RGD_2312511; RRID: SCR_002285; RRID: SCR_002815; RRID: SCR_002865; RRID: SCR_013674; RRID: SCR_014479; collybistin; gephyrin; in utero electroporation; synapse formation; vesicular GABA transporter

PMID:
27804142
DOI:
10.1002/cne.24137
[Indexed for MEDLINE]

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