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Cancer Biomark. 2017;18(1):11-17. doi: 10.3233/CBM-160667.

Serum microRNA-15a level acts as a potential diagnostic and prognostic biomarker for human esophageal squamous cell carcinoma.

Li J1,1, Li M2,1, Gao F2, Ge X2.

Author information

1
Department of Radiology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
2
Department of Radiology, Huadong Hospital Affliated to Fudan University, Shanghai, China.

Abstract

BACKGROUND:

Clinical significance of microRNA (miR)-15a in human esophageal squamous cell carcinoma (ESCC) remains unclear.

OBJECTIVE:

To evaluate the expression level of miR-15a and to determine its potential for diagnosis and prognosis in ESCC.

METHODS:

Quantitative reverse transcription polymerase chain reaction was performed to examine the expression levels of miR-15a in ESCC tissues and patients' sera. The diagnostic and prognostic implications of serum miR-15a level in human ESCC were further evaluated.

RESULTS:

Expression levels of miR-15a in ESCC tissues and patients' sera were significantly decreased (both P< 0.001). Additionally, serum miR-15a had an optimal diagnostic cut-off point (2.29) for ESCC with sensitivity of 86.36% and specificity of 100.00%. Moreover, low serum miR-15a level more frequently occurred in ESCC patients with advanced tumor-node-metastasis, T and N stages (all P= 0.01) and poor tumor differentiation (P= 0.03). The Kaplan-Meier curve showed that low miR-15a expression was significantly associated with shorter overall survival (OS) and disease-free survival (DFS) of ESCC patients (both P< 0.001). Further multivariate analysis identified miR-15a as an independent prognostic factor for both OS and DFS (both P= 0.01).

CONCLUSION:

Decreased expression of miR-15a may play a crucial role in ESCC development and progression. Serum miR-15a level could be used as a potential diagnostic and prognostic marker in clinics.

KEYWORDS:

Esophageal squamous cell carcinoma; clinicopathological feature; diagnosis; microRNA-15a; prognosis

PMID:
27802201
DOI:
10.3233/CBM-160667
[Indexed for MEDLINE]

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