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Front Plant Sci. 2016 Oct 17;7:1541. eCollection 2016.

Ectopic Expression of JcWRKY Transcription Factor Confers Salinity Tolerance via Salicylic Acid Signaling.

Author information

1
Plant Omics Division, Central Salt and Marine Chemicals Research Institute (CSIR) - Council of Scientific and Industrial Research Bhavnagar, India.
2
Plant Omics Division, Central Salt and Marine Chemicals Research Institute (CSIR) - Council of Scientific and Industrial ResearchBhavnagar, India; Academy of Scientific and Innovative Research, Central Salt and Marine Chemicals Research Institute -Council of Scientific and Industrial ResearchBhavnagar, India.

Abstract

Plants, being sessile, have developed intricate signaling network to specifically respond to the diverse environmental stress. The plant-specific WRKY TFs form one of the largest TF family and are involved in diverse plant processes, involving growth, development and stress signaling through auto and cross regulation with different genes and TFs. Here, we report the functional characterization of a salicylic acid -inducible JcWRKY TF. The JcWRKY overexpression confers salinity tolerance in transgenic tobacco, as was evident by increased chlorophyll content and seed germination potential. The transgenic plants showed increased soluble sugar, membrane stability, reduced electrolyte leakage and generation of reactive oxygen species (H2O2 and [Formula: see text]) as compared to the wild type. Furthermore, the low SA treatment along with salinity improved the tolerance potential of the transgenics by maintaining ROS homeostasis and high K+/Na+ ratio. The transcript expression of SA biosynthetic gene ICS1 and antioxidative enzymes (CAT and SOD) showed upregulation during stress. Thus, the present study reflects that JcWRKY is working in co-ordination with SA signaling to orchestrate the different biochemical and molecular pathways to maneuvre salt stress tolerance of the transgenic plants.

KEYWORDS:

Jatropha; JcWRKY; ROS homeostasis; phytohormones; salicylic acid; salinity; transcription factor(s); transgenic

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