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Rev Alerg Mex. 2016 Oct-Dec;63(4):365-372.

[Determination and analysis of protein profile of different transfer factors].

[Article in Spanish; Abstract available in Spanish from the publisher]

Author information

1
Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Laboratorio de Bioquímica Estructural. Ciudad de México, México. guillermoguidos@yahoo.com.

Abstract

in English, Spanish

BACKGROUND:

The transfer factor (TF) is the dialyzable extract of leukocytes with cellular immunity transfer properties. Its use has spread in the treatment of a wide range of immunologic, infectious, and even oncological diseases. However, important aspects in their protein profile, component concentrations, and a well-defined action mechanism are not completely unknown.

OBJECTIVES:

To analyze the protein profiles of different transfer factors marketed in Mexico.

METHODS:

6 TF marketed in Mexico were obtained and analyzed, quantifying protein with thaze Bradford method, by high-performance liquid chromatography (HPLC), and polyacrylamide gel electrophoresis (SDS-PAGE). All samples were analyzed in duplicate.

RESULTS:

The total protein concentrations of all TF analyzed are less than 0.2 mg/mL. The chromatographic profiles showed differences in some TF. The protein concentration was 6 to almost one thousand times lower compared to reports by some manufacturers.

CONCLUSION:

Almost all transfer factors marketed in Mexico lack a labeling and health record that meets the official standards.

KEYWORDS:

Protein analysis; Transfer factor

PMID:
27795217
DOI:
10.29262/ram.v63i4.190
[Indexed for MEDLINE]

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