Format

Send to

Choose Destination
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Dec 1;1038:63-72. doi: 10.1016/j.jchromb.2016.10.016. Epub 2016 Oct 14.

Semi-preparative HPLC separation followed by HPLC/UV and tandem mass spectrometric analysis of phorbol esters in Jatropha seed.

Author information

1
Department of Chemistry, Faculty of Natural Science, National University of Laos, Dongdok Campus, P.O. Box 7322, Xaythany District, Vientiane, Laos.
2
Danang Environmental Technology Center, Institute of Environmental Technology, Vietnam Academy of Science and Technology, Tran Dai Nghia Road, Ngu Hanh Son District, Danang, Vietnam.
3
Graduate School of Engineering, Osaka Prefecture University, 1-2 Gaken-cho, Naka-ku, Sakai-shi, Osaka 599-8531, Japan.
4
Research Organization for University-Community Collaborations, Osaka Prefecture University, 1-2 Gaken-cho, Naka-ku, Sakai-shi, Osaka 599-8531, Japan. Electronic address: k_imamura@riast.osakafu-u.ac.jp.
5
Research Organization for University-Community Collaborations, Osaka Prefecture University, 1-2 Gaken-cho, Naka-ku, Sakai-shi, Osaka 599-8531, Japan.
6
Faculty of Chemistry, Vietnam National University, 19 Le Thanh Tong St., Hanoi, Vietnam.

Abstract

Phorbol esters (PEs) are well known as the main toxic compounds in Jatropha curcas Linnaeus (JCL), the seed oil of which has been considered as a major feedstock for the production of biodiesel. In the present study, we investigated a series of PEs extracted from JCL seed kernels with methanol (MeOH), and identified more than seven components contained in the PEs. The isolation of main five components of a series of PEs was revised using a semi-preparative reversed phase HPLC analysis of ODS-3 column. The five peaks of components were successfully isolated, and peaks of J2, J3, J5, and J7 were assigned to be Jatropha factors C1, C2, C3, and C4/5, but J6 was a mixture of Jatropha factor C6 and its isomer based on the data of UV and LC-MS/MS, and J2 was identified using 1H NMR analysis. By characterization using LC-MS/MS analysis, all components of a series of PEs were elucidated to be the 12-deoxy-16-hydroxyphorbol esters composed of isomeric form of dicarboxylic groups with same m/z value of 380.

KEYWORDS:

(1)H NHR spectrometry; Jatropha curcas seeds; Phorbol ester isomers; Preparative HPLC; Reverse phase ODS-3 column; Tandem mass spectrometry characterization

PMID:
27794223
DOI:
10.1016/j.jchromb.2016.10.016
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center