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Anticancer Res. 2016 Nov;36(11):5821-5826.

Pulsed Electromagnetic Field with Temozolomide Can Elicit an Epigenetic Pro-apoptotic Effect on Glioblastoma T98G Cells.

Author information

1
Radiation Oncology Unit, Hospital San Matteo Foundation, Pavia, Italy.
2
Laboratory of Neuro-Radio-Experimental Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
3
Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, Italy.
4
Centre for Health Technologies, University of Pavia, Pavia, Italy.
5
Laboratory of Immunology and Genetic Analysis, Department of Earth and Environmental Sciences, University of Pavia, Pavia, Italy.
6
Laboratory of Neuro-Radio-Experimental Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, Italy enrica.capelli@unipv.it rosanna.nano@unipv.it.
7
Centre for Health Technologies, University of Pavia, Pavia, Italy enrica.capelli@unipv.it rosanna.nano@unipv.it.

Abstract

Treatment with pulsed electromagnetic fields (PEMFs) is emerging as an interesting therapeutic option for patients with cancer. The literature has demonstrated that low-frequency/low-energy electromagnetic fields do not cause predictable effects on DNA; however, they can epigenetically act on gene expression. The aim of the present work was to study a possible epigenetic effect of a PEMF, mediated by miRNAs, on a human glioblastoma cell line (T98G). We tested a PEMF (maximum magnetic induction, 2 mT; frequency, 75 Hz) that has been demonstrated to induce autophagy in glioblastoma cells. In particular, we studied the effect of PEMF on the expression of genes involved in cancer progression and a promising synergistic effect with temozolomide, a frequently used drug to treat glioblastoma multiforme. We found that electromagnetic stimulation in combination with temozolomide can elicit an epigenetic pro-apoptotic effect in the chemo- and radioresistant T98G glioblastoma cell line.

KEYWORDS:

Glioblastoma multiforme; epigenetic pro-apoptotic effect; pulsed electromagnetic stimulation; temozolomide

PMID:
27793904
DOI:
10.21873/anticanres.11166
[Indexed for MEDLINE]

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