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Bioresour Technol. 2017 Jan;223:149-156. doi: 10.1016/j.biortech.2016.10.032. Epub 2016 Oct 14.

Production of ε-poly-lysine by Streptomyces albulus PD-1 via solid-state fermentation.

Author information

1
State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
2
Fujian Inspecton and Research Institute for Food Quality, Fuzhou, Fujian 350108, China.
3
State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China. Electronic address: xuh@njtech.edu.cn.

Abstract

The aim of this study was to produce ε-poly-lysine (ε-PL) by Streptomyces albulus PD-1 through solid-state fermentation (SSF) using agro-industrial residues. Maximum ε-PL production (86.62mg/g substrate) was obtained a mixed substrate of rapeseed cake and wheat bran (2:1, w/w) supplemented with glucose (4%, w/w), (NH4)2SO4 (3%, w/w), with an initial moisture content of 65%, initial pH of 7.0 and inoculum size of 13% v/w, incubated at 30°C for 8days. The results of scanning electron microscopy indicated that the filamentous thallus could penetrate the substrate surface. Moreover, repeated-batch SSF was successfully conducted 8 times using 10% substrate as seeds for the next fermentation cycle, and the results suggest that repeated-batch SSF is more efficient because of the shortened lag phase. To the best of our knowledge, this is the first report on ε-PL production using the SSF process.

KEYWORDS:

Agro-industrial residues; Repeated-batch fermentation; Solid-state fermentation; Streptomyces albulus PD-1; ε-Poly-lysine

PMID:
27792924
DOI:
10.1016/j.biortech.2016.10.032
[Indexed for MEDLINE]

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