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Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6859-E6867. Epub 2016 Oct 17.

Dynamic translation regulation in Caulobacter cell cycle control.

Author information

1
Department of Developmental Biology, Stanford University, Stanford, CA 94305; shapiro@stanford.edu hmcadams@stanford.edu schrader@wayne.edu.
2
Department of Cellular and Molecular Pharmacology, California Institute of Quantitative Biology, Center for RNA Systems Biology, University of California, San Francisco, CA 94158.
3
Department of Developmental Biology, Stanford University, Stanford, CA 94305.
4
Howard Hughes Medical Institute, University of California, San Francisco, CA 94158.

Abstract

Progression of the Caulobacter cell cycle requires temporal and spatial control of gene expression, culminating in an asymmetric cell division yielding distinct daughter cells. To explore the contribution of translational control, RNA-seq and ribosome profiling were used to assay global transcription and translation levels of individual genes at six times over the cell cycle. Translational efficiency (TE) was used as a metric for the relative rate of protein production from each mRNA. TE profiles with similar cell cycle patterns were found across multiple clusters of genes, including those in operons or in subsets of operons. Collections of genes associated with central cell cycle functional modules (e.g., biosynthesis of stalk, flagellum, or chemotaxis machinery) have consistent but different TE temporal patterns, independent of their operon organization. Differential translation of operon-encoded genes facilitates precise cell cycle-timing for the dynamic assembly of multiprotein complexes, such as the flagellum and the stalk and the correct positioning of regulatory proteins to specific cell poles. The cell cycle-regulatory pathways that produce specific temporal TE patterns are separate from-but highly coordinated with-the transcriptional cell cycle circuitry, suggesting that the scheduling of translational regulation is organized by the same cyclical regulatory circuit that directs the transcriptional control of the Caulobacter cell cycle.

KEYWORDS:

Caulobacter; cell cycle; regulation; ribosome profiling; translation

PMID:
27791168
PMCID:
PMC5098616
DOI:
10.1073/pnas.1614795113
[Indexed for MEDLINE]
Free PMC Article

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