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Int J Nanomedicine. 2016 Oct 12;11:5041-5055. eCollection 2016.

Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells.

Author information

1
Department of Pharmaceutical and Pharmacological Sciences, University of Padova; Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS.
2
Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy.
3
Department of Pharmaceutical and Pharmacological Sciences, University of Padova.
4
Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS.

Abstract

Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality.

KEYWORDS:

ECM nanostructure signaling; TriCol decellularization procedure; blood-derived multipotent cells; guided tissue engineering; self-repopulation potential

PMID:
27789941
PMCID:
PMC5068475
DOI:
10.2147/IJN.S115999
[Indexed for MEDLINE]
Free PMC Article

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