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Development. 2016 Dec 1;143(23):4381-4393. Epub 2016 Oct 27.

Distinct capacity for differentiation to inner ear cell types by progenitor cells of the cochlea and vestibular organs.

McLean WJ1,2,3, McLean DT1,2, Eatock RA4, Edge AS5,2,3,6.

Author information

1
Department of Otology and Laryngology, Harvard Medical School, Boston, MA 02114, USA.
2
Eaton-Peabody Laboratories of Auditory Physiology, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA.
3
Program in Speech and Hearing Bioscience and Technology, Division of Health Sciences and Technology, Harvard & MIT, Cambridge, MA 02139, USA.
4
Department of Neurobiology, University of Chicago, Chicago, IL 60637, USA eatock@uchicago.edu albert_edge@meei.harvard.edu.
5
Department of Otology and Laryngology, Harvard Medical School, Boston, MA 02114, USA eatock@uchicago.edu albert_edge@meei.harvard.edu.
6
Harvard Stem Cell Institute, Cambridge, MA 02138, USA.

Abstract

Disorders of hearing and balance are most commonly associated with damage to cochlear and vestibular hair cells or neurons. Although these cells are not capable of spontaneous regeneration, progenitor cells in the hearing and balance organs of the neonatal mammalian inner ear have the capacity to generate new hair cells after damage. To investigate whether these cells are restricted in their differentiation capacity, we assessed the phenotypes of differentiated progenitor cells isolated from three compartments of the mouse inner ear - the vestibular and cochlear sensory epithelia and the spiral ganglion - by measuring electrophysiological properties and gene expression. Lgr5+ progenitor cells from the sensory epithelia gave rise to hair cell-like cells, but not neurons or glial cells. Newly created hair cell-like cells had hair bundle proteins, synaptic proteins and membrane proteins characteristic of the compartment of origin. PLP1+ glial cells from the spiral ganglion were identified as neural progenitors, which gave rise to neurons, astrocytes and oligodendrocytes, but not hair cells. Thus, distinct progenitor populations from the neonatal inner ear differentiate to cell types associated with their organ of origin.

KEYWORDS:

Cochlea; Hair cell; Mouse; Neural stem cell; Neuron; Spiral ganglion; Vestibular

PMID:
27789624
PMCID:
PMC5201044
DOI:
10.1242/dev.139840
[Indexed for MEDLINE]
Free PMC Article

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