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J Gerontol A Biol Sci Med Sci. 2017 Sep 1;72(9):1163-1170. doi: 10.1093/gerona/glw216.

Cytomegalovirus Seropositivity Predicts a Decline in the T Cell But Not the Antibody Response to Influenza in Vaccinated Older Adults Independent of Type 2 Diabetes Status.

Author information

1
Health Sciences North Research Institute, Sudbury, ON, Canada.
2
Research Center on Aging, Université de Sherbrooke, Sherbrooke, QC, Canada.
3
Department of Medicine, University of British Columbia, Vancouver, Canada.
4
University of Connecticut Center on Aging, Farmington.
5
Second Department of Internal Medicine, University of Tübingen Medical School, Tübingen, Germany.
6
School of Science and Technology, College of Arts and Science, Nottingham Trent University, Nottingham, UK.

Abstract

Type 2 diabetes mellitus (T2DM) and persistent cytomegalovirus (CMV) infection are postulated contributors to inflammatory processes that impact on the age-related decline in T-cell responses to influenza vaccination. Older subjects with T2DM (n = 30) and healthy aged controls (n = 40) were enrolled and received influenza vaccination in this study. Serum inflammatory markers and CMV serostatus were measured. Pre- to post-vaccination changes in serum antibody titers to the A/H3N2 strain, and levels of granzyme B (GrB, cytotoxic T lymphocytes) in lysates and cytokines in supernatants from influenza A/H3N2-challenged peripheral blood mononuclear cells were measured. We found no difference between the T2DM and healthy groups in the immune responses measured. However, CMV serostatus was a key determinant of the GrB response to influenza challenge; CMV+ subjects had low levels of inducible GrB (iGrB) activity in response to influenza challenge. In contrast, the serum antibody response to the A/H3N2 vaccine strain did not differ with CMV serostatus, and serum levels of the inflammatory marker, β2-microglobulin, were positively correlated with age, T2DM, and serum IL-10 levels. In conclusion, CMV seropositivity associated with a decline in GrB responses to influenza may predict increased susceptibility to influenza in older adults.

KEYWORDS:

Cytomegalovirus (CMV); Granzyme B; Influenza vaccination; Older adults; T cell

PMID:
27789617
PMCID:
PMC5861868
DOI:
10.1093/gerona/glw216
[Indexed for MEDLINE]
Free PMC Article

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