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Methods Mol Biol. 2017;1493:277-286.

Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot-Conjugated Proteins.

Author information

1
Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
2
Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. deanna.benson@mssm.edu.

Abstract

Neurons can endocytose soluble semaphorins to either initiate or interrupt signaling at the cell membrane. Depending on the cell type and even on the specific subcellular domain, the endocytic process will differ in intensity, speed, and modality, and will subsequently facilitate diverse actions of semaphorin molecules. Therefore, in order to understand the physiology of guidance cues like semaphorins it is important to visualize endocytic events with good spatial and temporal resolution. Here, we describe methods to visualize endocytosed Semaphorin3A (Sema3A) molecules and to characterize the rate and pathway of internalization in primary rat neuronal cultures using semiconductor quantum dot nanoparticles (Q-dots).

KEYWORDS:

Acid strip; Endocytosis; Microscopy; Q-dots; Semaphorin3A

PMID:
27787858
DOI:
10.1007/978-1-4939-6448-2_20
[Indexed for MEDLINE]

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