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Appl Plant Sci. 2016 Oct 13;4(10). pii: apps.1600072. eCollection 2016 Oct.

Characterization of polymorphic microsatellite markers in Pinus armandii (Pinaceae), an endemic conifer species to China.

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1
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.

Abstract

PREMISE OF THE STUDY:

Pinus armandii (Pinaceae) is an important conifer tree species in central and southwestern China, and it plays a key role in the local forest ecosystems. To investigate its population genetics and design effective conservation strategies, we characterized 18 polymorphic microsatellite markers for this species.

METHODS AND RESULTS:

Eighteen novel polymorphic and 16 monomorphic microsatellite loci of P. armandii were isolated using Illumina MiSeq technology. The number of alleles per locus ranged from two to five. The expected heterozygosity ranged from 0.061 to 0.609 with an average of 0.384, and the observed heterozygosity ranged from 0.063 to 0.947 with an average of 0.436. Seventeen loci could be successfully transferred to five related Pinus species (P. koraiensis, P. griffithii, P. sibirica, P. pumila, and P. bungeana).

CONCLUSIONS:

These novel microsatellites could potentially be used to investigate the population genetics of P. armandii and related species.

KEYWORDS:

Pinaceae; Pinus armandii; cross-amplification; microsatellite markers; polymorphism; population genetics

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