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Nanomedicine (Lond). 2016 Nov;11(22):2889-2902. Epub 2016 Oct 26.

Differential uptake of nanoparticles by human M1 and M2 polarized macrophages: protein corona as a critical determinant.

Author information

1
Department of Biomaterials Science & Technology, Targeted Therapeutics Section, MIRA Institute for Biomedical Technology & Technical Medicine, University of Twente, Enschede, The Netherlands.
2
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.

Abstract

AIM:

To investigate the interaction behavior of M1- and M2-type macrophages with nanoparticles of different sizes with/without the presence of serum.

MATERIALS & METHODS:

THP-1 human monocytes were differentiated into M1 and M2 macrophages, and the uptake of silica nanoparticle (50-1000 nm) was studied using flow cytometry and different microscopies.

RESULTS:

Without serum, higher uptake of all-sized nanoparticles was observed by M1 compared with M2. With serum, uptake of nanoparticles (200-1000 nm) was dramatically increased by M2. Furthermore, serum proteins adsorbed (corona) by nanoparticles were found to be the ligands for receptors expressed by M2, as revealed by SDS-PAGE and gene profiling analyses.

CONCLUSION:

The observed differential uptake by M1 and M2 macrophages will help understand the fate of nanoparticles in vivo.

KEYWORDS:

M1 macrophages; M2 macrophages; complement factor; heat-inactivated serum; nanoparticles; opsonization; phagocytosis; protein corona; serum proteins; silica nanoparticles

PMID:
27780415
DOI:
10.2217/nnm-2016-0233
[Indexed for MEDLINE]

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