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J Agric Food Chem. 2016 Nov 16;64(45):8516-8524. Epub 2016 Nov 2.

Characterization and Quantitation of Vitamin B12 Compounds in Various Chlorella Supplements.

Author information

1
Faculty of Agriculture, School of Agricultural, Biological, and Environmental Sciences, Tottori University , Tottori 680-8553 Japan.
2
Graduate School of Life and Environmental Sciences, Osaka Prefecture University , Osaka 598-8531 Japan.
3
Sun Chlorella Corporation , Kyoto 600-8177 Japan.

Abstract

Vitamin B12 was determined and characterized in 19 dried Chlorella health supplements. Vitamin contents of dried Chlorella cells varied from <0.1 μg to approximately 415 μg per 100 g of dry weight. Subsequent liquid chromatography/electrospray ionization-tandem mass spectrometry analyses showed the presence of inactive corrinoid compounds, a cobalt-free corrinoid, and 5-methoxybenzimidazolyl cyanocobamide (factor IIIm) in four and three high vitamin B12-containing Chlorella tablets, respectively. In four Chlorella tablet types with high and moderate vitamin B12 contents, the coenzyme forms of vitamin B12 5'-deoxyadenosylcobalamin (approximately 32%) and methylcobalamin (approximately 8%) were considerably present, whereas the unnaturally occurring corrinoid cyanocobalamin was present at the lowest concentrations. The species Chlorella sorokiniana (formerly Chlorella pyrenoidosa) is commonly used in dietary supplements and did not show an absolute requirement of vitamin B12 for growth despite vitamin B12 uptake from the medium being observed. In further experiments, vitamin B12-dependent methylmalonyl-CoA mutase and methionine synthase activities were detected in cell homogenates. In particular, methionine synthase activity was significantly increased following the addition of vitamin B12 to the medium. These results suggest that vitamin B12 contents of Chlorella tablets reflect the presence of vitamin B12-generating organic ingredients in the medium or the concomitant growth of vitamin B12-synthesizing bacteria under open culture conditions.

KEYWORDS:

Chlorella sorokiniana; Chlorella tablets; vitamin B12

PMID:
27776413
DOI:
10.1021/acs.jafc.6b03550
[Indexed for MEDLINE]

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