Format

Send to

Choose Destination
Nat Methods. 2016 Dec;13(12):985-988. doi: 10.1038/nmeth.4034. Epub 2016 Oct 24.

Bright photoactivatable fluorophores for single-molecule imaging.

Author information

1
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.

Abstract

Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.

PMID:
27776112
DOI:
10.1038/nmeth.4034
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center