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Sci Rep. 2016 Oct 24;6:35382. doi: 10.1038/srep35382.

'Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci.

Author information

1
Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Paseo Río Linares s/n 33300- Villaviciosa, Asturias, Spain.
2
Laboratory of Gene Technology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 21 - box 2462, 3001 Heverlee, Belgium.
3
Lisando GmbH, Am BioPark 13, 93053 Regensburg, Germany.
4
Laboratory of Food Microbiology, Department of Microbial and Molecular Systems, KU Leuven, Heverlee, Belgium.
5
Laboratory of Applied Biotechnology, Department of Applied Biosciences, Ghent University, Ghent Belgium.

Abstract

Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10 min. In addition, lower doses of Art-240 are required to achieve the same bactericidal effect.

PMID:
27775093
PMCID:
PMC5075790
DOI:
10.1038/srep35382
[Indexed for MEDLINE]
Free PMC Article

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