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Protein Sci. 2017 Feb;26(2):365-374. doi: 10.1002/pro.3073. Epub 2016 Nov 2.

The macro domain as fusion tag for carrier-driven crystallization.

Author information

1
Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Switzerland.

Abstract

Obtaining well-ordered crystals remains a significant challenge in protein X-ray crystallography. Carrier-driven crystallization can facilitate crystal formation and structure solution of difficult target proteins. We obtained crystals of the small and highly flexible SPX domain from the yeast vacuolar transporter chaperone 4 (Vtc4) when fused to a C-terminal, non-cleavable macro tag derived from human histone macroH2A1.1. Initial crystals diffracted to 3.3 Å resolution. Reductive protein methylation of the fusion protein yielded a new crystal form diffracting to 2.1 Å. The structures were solved by molecular replacement, using isolated macro domain structures as search models. Our findings suggest that macro domain tags can be employed in recombinant protein expression in E. coli, and in carrier-driven crystallization.

KEYWORDS:

carrier-driven crystallization; crystallization tag; histone macroH2A; macro domain; recombinant protein expression

PMID:
27774698
PMCID:
PMC5275734
DOI:
10.1002/pro.3073
[Indexed for MEDLINE]
Free PMC Article

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