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Reprod Toxicol. 2016 Dec;66:107-114. doi: 10.1016/j.reprotox.2016.10.004. Epub 2016 Oct 20.

Effects of isoliquiritigenin on ovarian antral follicle growth and steroidogenesis.

Author information

1
Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: mahalin2@illinois.edu.
2
Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: lgao@illinois.edu.
3
Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: jeisner2@illinois.edu.
4
Department of Food Science and Human Nutrition, University of Illinois, 905 S. Goodwin, Urbana, IL 61801, United States. Electronic address: helferic@illinois.edu.
5
Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: jflaws@illinois.edu.

Abstract

Isoliquiritigenin is a botanical estrogen used as a dietary supplement. Previous studies show that other botanical estrogens affect ovarian estradiol synthesis, but isoliquiritigenin's effects on the ovary are unknown. Thus, this study tested the hypothesis that isoliquiritigenin inhibits ovarian antral follicle growth and steroidogenesis. Antral follicles from CD-1 mice were cultured with vehicle control (dimethyl sulfoxide; DMSO) or isoliquiritigenin (0.6μM, 6 μM, 36 μM, and 100 μM) for 48-96h. During culture, follicle diameters were measured daily to assess follicle growth. After culture, media were collected for hormone assays and follicles were collected for gene expression analysis of steroidogenic enzymes. Isoliquiritigenin inhibited antral follicle growth and altered estradiol, testosterone, and progesterone levels. Additionally, isoliquiritigenin altered the mRNA levels of cytochrome P450 steroid 17-α-hydroxylase 1, aromatase, 17β-hydroxysteroid dehydrogenase 1, and steroidogenic acute regulatory protein. These data indicate that exposure to isoliquiritigenin inhibits growth and disrupts steroid production in antral follicles.

KEYWORDS:

Antral follicle; Isoliquiritigenin; Licorice root; Ovary; Steroidogenesis

PMID:
27773742
PMCID:
PMC5125911
DOI:
10.1016/j.reprotox.2016.10.004
[Indexed for MEDLINE]
Free PMC Article

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