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Diagn Microbiol Infect Dis. 2017 Jan;87(1):64-67. doi: 10.1016/j.diagmicrobio.2016.10.001. Epub 2016 Oct 7.

High-virulence CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from commercial turkeys.

Author information

1
Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, College of Veterinary Medicine and Zootechny, São Paulo, Brazil.
2
Department of Pathology, University of São Paulo, College of Veterinary Medicine and Zootechnics, São Paulo, Brazil.
3
Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
4
Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
5
Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, College of Veterinary Medicine and Zootechny, São Paulo, Brazil. Electronic address: morenoam@usp.br.

Abstract

This study reports the high-virulence phylogenetic backgrounds of CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from turkeys sent to slaughter and condemned by airsacculitis in Brazil. Among 300 air sac samples, seven E. coli strains produced plasmid-mediated CMY-2-type AmpC, of which three carried also the blaCTX-M-2 Extended Spectrum Beta-Lactamase encoding gene. Interestingly, the transfer of the blaCMY-2 gene was positive for three E. coli strains, being associated with the presence of IncI1 plasmids. The complete sequence of the representative pJB10 plasmid revealed that the blaCMY-2 gene was within a transposon-like element in the classical genetic environment consisting of tnpA-blaCMY-2-blc-sugE structure. This plasmid with 94-kb belonged to the sequence type (ST) 12 among IncI1 plasmids, which has been associated with the worldwide spread of blaCMY-2 among Salmonella enterica and E. coli. Furthermore, to the best of our knowledge, this is the first complete sequence of a CMY-2-encoding plasmid derived from an Escherichia coli isolated from food-producing animals in Latin America.

KEYWORDS:

CMY-2; ESBL; IncI1/ST12; Plasmid; Turkey

[Indexed for MEDLINE]

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