Format

Send to

Choose Destination
Arch Oral Biol. 2017 Jan;73:142-150. doi: 10.1016/j.archoralbio.2016.10.010. Epub 2016 Oct 15.

Insulin-like growth factor 1 (IGF1) affects proliferation and differentiation and wound healing processes in an inflammatory environment with p38 controlling early osteoblast differentiation in periodontal ligament cells.

Author information

1
Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Welschnonnenstr. 17, 53111 Bonn, Germany; Department of Orthodontics, University of Bonn, Bonn, Germany; Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany; Department of Oral & Maxillofacial Plastic Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany.
2
Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Welschnonnenstr. 17, 53111 Bonn, Germany.
3
Department of Orthodontics, University of Bonn, Bonn, Germany.
4
Department of Oral & Maxillofacial Plastic Surgery, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany.
5
Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany. Electronic address: jochen.winter@ukb.uni-bonn.de.

Abstract

OBJECTIVE:

The objective of this study was to investigate effects of insulin-like growth factor 1 (IGF1) on proliferation, wound healing and differentiation processes of human periodontal ligament (PDL) cells under inflammatory conditions and whether the protective, anabolic effects of IGF1 can attenuate unfavorable effects of interleukin-1β (IL-1β).

DESIGN:

Inflammation was mimicked through cell stimulation with IL-1β. PDL cells were characterized in respect to the presence of components of the IGF system and the responsive potential on IL-1β incubation. Gene expression levels were analyzed by quantitative real-time PCR. Cellular localization of target proteins was visualized using fluorescent-based immunohistochemistry. Effects on cell division were investigated by proliferation assays. Wound healing was analyzed using light microscopic techniques. Differentiation was quantified by measuring biomineralization and osteoblast-specific alkaline phosphatase enzyme activity.

RESULTS:

PDL cell proliferation and wound healing were positively affected by IGF1 and the combination of IGF1 with IL-1β, while only IL-1β showed negative effects. Biomineralization was enhanced by IGF1, IL-1β, and the combination of both stimulants. Osteoblast differentiation was increased by IL-1β and the combination of IL-1β with IGF1, whereas only IGF1 negatively affected ALP activity. Phosphorylation of p38 was regulated by IL-1β and IGF1.

CONCLUSIONS:

The data presented in this work showed a potential of IGF1 to improve wound healing and proliferation processes and to sustain cell differentiation under inflammatory stimuli in PDL cells.

KEYWORDS:

Biomineralization; IGF-1; IL-1β; Inflammation; p38

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center