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J Integr Plant Biol. 2016 Dec;58(12):943-946. doi: 10.1111/jipb.12502. Epub 2016 Nov 23.

Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution.

Author information

1
Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
2
University of Chinese Academy of Sciences, Beijing 100049, China.
3
Departments of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland.

Abstract

We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H2 ) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species (ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo-oxidative damage and thereby ensures electron supply to the hydrogenase.

KEYWORDS:

Chlamydomonas reinhardtii; hpm91; hydrogen photoproduction; oxidative stress; photosystem II; sulfur deprivation

PMID:
27762070
DOI:
10.1111/jipb.12502
[Indexed for MEDLINE]

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