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J Biomed Mater Res B Appl Biomater. 2017 Nov;105(8):2612-2617. doi: 10.1002/jbm.b.33804. Epub 2016 Oct 19.

Antimicrobial activity of immobilized lactoferrin and lactoferricin.

Author information

1
School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
2
School of Chemistry, University of New South Wales, Sydney, Australia.
3
School of Mathematical and Physical Sciences, University of Technology, Sydney, Australia.

Abstract

Lactoferrin and lactoferricin were immobilized on glass surfaces via two linkers, 4-azidobenzoic acid (ABA) or 4-fluoro-3-nitrophenyl azide (FNA). The resulting surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The antimicrobial activity of the surfaces was determined using Pseudomonas aeruginosa and Staphylococcus aureus strains by fluorescence microscopy. Lactoferrin and lactoferricin immobilization was confirmed by XPS showing significant increases (p < 0.05) in nitrogen on the glass surface. The immobilization of both proteins slightly increased the overall hydrophobicity of the glass. Both lactoferrin and lactoferricin immobilized on glass significantly (p < 0.05) reduced the numbers of viable bacterial cells adherent to the glass. For P. aeruginosa, the immobilized proteins consistently increased the percentage of dead cells compared to the total cells adherent to the glass surfaces (p < 0.03). Lactoferrin and lactoferricin were successfully immobilized on glass surfaces and showed promising antimicrobial activity against pathogenic bacteria.

KEYWORDS:

Pseudomonas; Staphylococcus; antimicrobial; bacterial adherence; lactoferricin; lactoferrin

PMID:
27758034
DOI:
10.1002/jbm.b.33804
[Indexed for MEDLINE]

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