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Mol Cell Proteomics. 2016 Dec;15(12):3653-3664. Epub 2016 Oct 17.

Salmonella Typhimurium Enzymatically Landscapes the Host Intestinal Epithelial Cell (IEC) Surface Glycome to Increase Invasion.

Author information

1
From the ‡Department of Chemistry, University of California, Davis, CA, 95616.
2
§Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, 95616.
3
¶Department of Dermatology, University of California, Davis School of Medicine, Sacramento, CA, 95817.
4
From the ‡Department of Chemistry, University of California, Davis, CA, 95616; cblebrilla@ucdavis.edu.

Abstract

Although gut host-pathogen interactions are glycan-mediated processes, few details are known about the participating structures. Here we employ high-resolution mass spectrometric profiling to comprehensively identify and quantitatively measure the exact modifications of native intestinal epithelial cell surface N-glycans induced by S. typhimurium infection. Sixty minutes postinfection, select sialylated structures showed decreases in terms of total number and abundances. To assess the effect of cell surface mannosylation, we selectively rerouted glycan expression on the host using the alpha-mannosidase inhibitor, kifunensine, toward overexpression of high mannose. Under these conditions, internalization of S. typhimurium significantly increased, demonstrating that bacteria show preference for particular structures. Finally, we developed a novel assay to measure membrane glycoprotein turnover rates, which revealed that glycan modifications occur by bacterial enzyme activity rather than by host-derived restructuring strategies. This study is the first to provide precise structural information on how host N-glycans are altered to support S. typhimurium invasion.

PMID:
27754876
PMCID:
PMC5141278
DOI:
10.1074/mcp.M116.063206
[Indexed for MEDLINE]
Free PMC Article

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