New member of the hormone-sensitive lipase family from the permafrost microbial community

Bioengineered. 2017 Jul 4;8(4):420-423. doi: 10.1080/21655979.2016.1230571. Epub 2016 Oct 18.

Abstract

Siberian permafrost is a unique environment inhabited with diverse groups of microorganisms. Among them, there are numerous producers of biotechnologically relevant enzymes including lipases and esterases. Recently, we have constructed a metagenomic library from a permafrost sample and identified in it several genes coding for potential lipolytic enzymes. In the current work, properties of the recombinant esterases obtained from this library are compared with the previously characterized lipase from Psychrobacter cryohalolentis and other representatives of the hormone-sensitive lipase family.

Keywords: HSL family; Siberian permafrost; esterase; metagenomic library.

MeSH terms

  • Enzyme Activation
  • Enzyme Stability
  • Metagenome / genetics*
  • Microbial Consortia / physiology*
  • Permafrost / microbiology*
  • Siberia
  • Sterol Esterase / chemistry*
  • Sterol Esterase / genetics*

Substances

  • Sterol Esterase