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Calcif Tissue Int. 2017 Jan;100(1):80-86. doi: 10.1007/s00223-016-0197-4. Epub 2016 Oct 17.

Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.

Author information

1
Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), MOVE Research Institute, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081LA, Amsterdam, The Netherlands. a.bronckers@acta.nl.
2
Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), MOVE Research Institute, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081LA, Amsterdam, The Netherlands.
3
Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute and Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, AB, T2N 4Z6, Canada.

Abstract

Exposure of forming enamel to fluoride results into formation of hypomineralized enamel. We tested whether enamel hypomineralization was caused by lower expression of the NCKX4/SLC24A4 Ca2+-transporter by ameloblasts. Three commercial antibodies against NCKX4 were tested on enamel organs of wild-type and Nckx4-null mice, one of which (a mouse monoclonal) was specific. This antibody gave a prominent staining of the apical plasma membranes of maturation ameloblasts, starting at early maturation. The layer of immuno-positive ameloblasts contained narrow gaps without immunostaining or with reduced staining. In fluorotic mouse incisors, the quantity of NCKX4 protein in ameloblasts as assessed by western blotting was not different from that in non-fluorotic ameloblasts. However, immunostaining of the apical plasma membranes of fluorotic ameloblasts was strongly reduced or absent suggesting that trafficking of NCKX4 to the apical membrane was strongly reduced. Exposure to fluoride may reduce NCKX4-mediated transport of Ca2+ by maturation stage ameloblasts which delays ameloblast modulation and reduces enamel mineralization.

KEYWORDS:

Calcium; Enamel fluorosis; Mechanism; Null mutation; Transport

PMID:
27752731
PMCID:
PMC5215084
DOI:
10.1007/s00223-016-0197-4
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

A. L. J. J. Bronckers, R. Jalali, J. Lytton declare that they have no conflict of interest. Human and Animal Rights and Informed Consent The authors confirm that the animal studies were approved and carried out according to national guidelines.

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