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Elife. 2016 Oct 18;5. pii: e18716. doi: 10.7554/eLife.18716.

Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding.

Author information

1
Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A*STAR, Singapore, Singapore.
2
Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States.
3
Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, United States.
4
Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, United States.

Abstract

Multiple hypothalamic neuronal populations that regulate energy balance have been identified. Although hypothalamic glia exist in abundance and form intimate structural connections with neurons, their roles in energy homeostasis are less known. Here we show that selective Ca2+ activation of glia in the mouse arcuate nucleus (ARC) reversibly induces increased food intake while disruption of Ca2+ signaling pathway in ARC glia reduces food intake. The specific activation of ARC glia enhances the activity of agouti-related protein/neuropeptide Y (AgRP/NPY)-expressing neurons but induces no net response in pro-opiomelanocortin (POMC)-expressing neurons. ARC glial activation non-specifically depolarizes both AgRP/NPY and POMC neurons but a strong inhibitory input to POMC neurons balances the excitation. When AgRP/NPY neurons are inactivated, ARC glial activation fails to evoke any significant changes in food intake. Collectively, these results reveal an important role of ARC glia in the regulation of energy homeostasis through its interaction with distinct neuronal subtype-specific pathways.

KEYWORDS:

arcuate nucleus; feeding; glial cell; mouse; neuroscience

PMID:
27751234
PMCID:
PMC5068968
DOI:
10.7554/eLife.18716
[Indexed for MEDLINE]
Free PMC Article

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