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Chem Commun (Camb). 2016 Oct 27;52(88):13024-13026.

Site specific protein O-glucosylation with bacterial toxins.

Author information

1
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.

Abstract

Using a MALDI-MS based assay, the kinetic parameters for peptide glucosylation using the C. difficile toxin B glycosyltransferase domain were determined. The minimum consensus sequence for glucosylation was YXXTXFXXY and the optimal peptide found was YAPTVFDAY. Using this sequence, homogenous glucosylated proteins could be readily produced.

PMID:
27748773
DOI:
10.1039/c6cc06223g
[Indexed for MEDLINE]

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