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Nat Biotechnol. 2016 Nov;34(11):1198-1205. doi: 10.1038/nbt.3681. Epub 2016 Oct 17.

A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti.

Author information

1
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
2
Department of Agronomy, University of Wisconsin-Madison, Madison, Wisconsin, USA.
3
Department of Computer Sciences, University of Wisconsin-Madison, Madison, Wisconsin, USA.
4
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
5
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
6
Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
7
Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA.
8
Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Abstract

Legumes are essential components of agricultural systems because they enrich the soil in nitrogen and require little environmentally deleterious fertilizers. A complex symbiotic association between legumes and nitrogen-fixing soil bacteria called rhizobia culminates in the development of root nodules, where rhizobia fix atmospheric nitrogen and transfer it to their plant host. Here we describe a quantitative proteomic atlas of the model legume Medicago truncatula and its rhizobial symbiont Sinorhizobium meliloti, which includes more than 23,000 proteins, 20,000 phosphorylation sites, and 700 lysine acetylation sites. Our analysis provides insight into mechanisms regulating symbiosis. We identify a calmodulin-binding protein as a key regulator in the host and assign putative roles and targets to host factors (bioactive peptides) that control gene expression in the symbiont. Further mining of this proteomic resource may enable engineering of crops and their microbial partners to increase agricultural productivity and sustainability.

PMID:
27748755
DOI:
10.1038/nbt.3681
[Indexed for MEDLINE]

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