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Macromol Biosci. 2017 Mar;17(3). doi: 10.1002/mabi.201600178. Epub 2016 Oct 17.

The Synergistic Effect of Cationic Moieties and GRGDSF-Peptides in Hydrogels on Neural Stem Cell Behavior.

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Faculty of Chemistry and Chemical Biology, Otto-Hahn Str. 6, TU Dortmund, 44227, Dortmund, Germany.
Department of Cell Morphology and Molecular Neurobiology, Ruhr-University Bochum, 44801, Bochum, Germany.
Dortmunder Elektronenspeicherringanlage (DELTA), Maria-Goeppert-Meyer Str. 2, TU Dortmund, 44227, Dortmund, Germany.


This article reports the behavior of embryonic neural stem cells on a hydrogel that combines cationic, non-specific cell adhesion motifs with glycine-arginine-glycine-aspartic acid-serine-phenylalanine (GRGDSF)-peptides as specific cell adhesion moieties. Therefore, three hydrogels are prepared by free radical polymerization that contains either a GRGDSF-peptide residue (P1), amino ethylmethacrylate as a cationic residue (P2), or a combination of both motifs (P3). For each gel, cross linker concentrations of 8 mol% is used to have a comparable gel stiffness of 8-9 kPa. The cell experiments indicate a synergistic effect of the non-specific, cationic residues, and the specific GRGDSF-peptides on embryonic neural stem cell behavior that is especially pronounced in the cell adhesion experiments by more than doubling the number of cells after 72 h when comparing P3 with P2 and is less pronounced in the proliferation and differentiation experiments.


NSC behavior 2D cell experiments; RGD-peptide; cationic hydrogels; embryonic NSCs

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