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Bioresour Technol. 2016 Dec;222:517-522. doi: 10.1016/j.biortech.2016.09.120. Epub 2016 Oct 1.

Consolidated bioprocessing of microalgal biomass to carboxylates by a mixed culture of cow rumen bacteria using anaerobic sequencing batch reactor (ASBR).

Author information

1
Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA.
2
Rural Energy Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
3
Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA.
4
Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA.
5
Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA. Electronic address: chens@wsu.edu.

Abstract

This study employed mixed-culture consolidated bioprocessing (CBP) to digest microalgal biomass in an anaerobic sequencing batch reactor (ASBR). The primary objectives are to evaluate the impact of hydraulic residence time (HRT) on the productivity of carboxylic acids and to characterize the bacterial community. HRT affects the production rate and patterns of carboxylic acids. For the 5-L laboratory-scale fermentation, a 12-day HRT was selected because it offered the highest productivity of carboxylic acids and it synthesized longer chains. The variability of the bacterial community increased with longer HRT (R2=0.85). In the 5-L laboratory-scale fermentor, the most common phyla were Firmicutes (58.3%), Bacteroidetes (27.4%), and Proteobacteria (11.9%). The dominant bacterial classes were Clostridia (29.8%), Bacteroidia (27.4%), Tissierella (26.2%), and Betaproteobacteria (8.9%).

KEYWORDS:

ASBR reactor; Algal biomass; Carboxylates; Consolidated bioprocessing; Cow rumen bacteria

PMID:
27743774
DOI:
10.1016/j.biortech.2016.09.120
[Indexed for MEDLINE]

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