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Elife. 2016 Oct 14;5. pii: e15983. doi: 10.7554/eLife.15983.

The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled.

Gupta T1,2,3,4, Kumar A1,2,3,4, Cattenoz PB1,2,3,4, VijayRaghavan K5,6, Giangrande A1,2,3,4.

Author information

1
Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
2
Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.
3
Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France.
4
Université de Strasbourg, Illkirch, France.
5
Department of Developmental Biology and Genetics, Tata Institute for Fundamental Research, Bangalore, India.
6
National Centre for Biological Sciences, Tata Institute for Fundamental Research, Bangalore, India.

Abstract

Collective migration is a complex process that contributes to build precise tissue and organ architecture. Several molecules implicated in cell interactions also control collective migration, but their precise role and the finely tuned expression that orchestrates this complex developmental process are poorly understood. Here, we show that the timely and threshold expression of the Netrin receptor Frazzled triggers the initiation of glia migration in the developing Drosophila wing. Frazzled expression is induced by the transcription factor Glide/Gcm in a dose-dependent manner. Thus, the glial determinant also regulates the efficiency of collective migration. NetrinB but not NetrinA serves as a chemoattractant and Unc5 contributes as a repellant Netrin receptor for glia migration. Our model includes strict spatial localization of a ligand, a cell autonomously acting receptor and a fate determinant that act coordinately to direct glia toward their final destination.

KEYWORDS:

D. melanogaster; Drosophila; collective cell migration; developmental biology; frazzled; glial cell; glide/gcm; stem cells

PMID:
27740455
PMCID:
PMC5114015
DOI:
10.7554/eLife.15983
[Indexed for MEDLINE]
Free PMC Article

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