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Asian-Australas J Anim Sci. 2017 Mar;30(3):432-438. doi: 10.5713/ajas.16.0391. Epub 2016 Oct 5.

Effects of low NaNO2 and NaCl concentrations on Listeria monocytogenes growth in emulsion-type sausage.

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Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea.
National Institute of Animal Science, RDA, Wanju 55365, Korea.
Department of Oral Mcirobiology, College of Dentistry, Wonkwang University, Iksan 54538, Korea.



The objective of this study was to evaluate the effect of combinations of NaNO2 and NaCl concentrations on Listeria monocytogenes (L. monocytogenes) growth in emulsion-type sausage.


Emulsion-type sausages formulated with different combinations of NaNO2 (0 and 10 ppm) and NaCl (1.00%, 1.25%, and 1.50%) were inoculated with a five-strain L. monocytogenes mixture, and stored at 4°C, 10°C, and 15°C, under aerobic or vacuum conditions. L. monocytogenes cell counts were measured at appropriate intervals, and kinetic parameters such as growth rate and lag phase duration (LPD) were calculated using the modified Gompertz model.


Growth rates increased (0.004 to 0.079 Log colony-forming unit [CFU]/g/h) as storage temperature increased, but LPD decreased (445.11 to 8.35 h) as storage temperature and NaCl concentration increased. The effect of combinations of NaCl and low-NaNO2 on L. monocytogenes growth was not observed at 4°C and 10°C, but it was observed at 15°C, regardless of atmospheric conditions.


These results indicate that low concentrations of NaNO2 and NaCl in emulsion-type sausage may not be sufficient to prevent L. monocytogenes growth, regardless of whether they are vacuum-packaged and stored at low temperatures. Therefore, additional techniques are necessary for L. monocytogenes control in the product.


Emulsion-type Sausage; Growth Pattern; Listeria monocytogenes; NaCl; NaNO2

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