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J Infect Dis. 2016 Dec 15;214(12):1884-1892. Epub 2016 Oct 12.

Identification of Chlamydia trachomatis Antigens Recognized by T Cells From Highly Exposed Women Who Limit or Resist Genital Tract Infection.

Author information

1
Department of Pediatrics.
2
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh School of Medicine and Magee-Womens Research Institute, Pennsylvania.
3
Department of Epidemiology and Biostatistics, Texas A&M Health Science Center, College Station.
4
Genocea Biosciences, Cambridge, Massachusetts.
5
Department of Biostatistics, University of North Carolina-Chapel Hill.

Abstract

BACKGROUND:

 Natural infection induces partial immunity to Chlamydia trachomatis Identification of chlamydial antigens that induce interferon γ (IFN-) secretion by T cells from immune women could advance vaccine development.

METHODS:

 IFN-γ production by CD4+ and CD8+ peripheral blood T cells from 58 high-risk women was measured after coculture with antigen-presenting cells preincubated with recombinant Escherichia coli expressing a panel of 275 chlamydial antigens. Quantile median regression analysis was used to compare frequencies of IFN-γ responses in women with only cervical infection to those in women with endometrial infection and frequencies in women who remained uninfected for over 1 year to those in women who developed incident infection. Statistical methods were then used to identify proteins associated with protection.

RESULTS:

 A higher median frequency of CD8+ T-cell responses was detected in women with lower genital tract chlamydial infection, compared with those with upper genital tract chlamydial infection (13.8% vs 9.5%; P =04), but the CD4+ T-cell response frequencies were not different. Women who remained uninfected displayed a greater frequency of positive CD4+ T-cell responses (29% vs 18%; P < .0001), compared with women who had incident infection, while the frequencies of CD8+ T-cell responses did not differ. A subset of proteins involved in central metabolism, type III secretion, and protein synthesis were associated with protection.

CONCLUSIONS:

 Investigations in naturally exposed women reveal protective responses and identify chlamydial vaccine candidate antigens.

KEYWORDS:

Chlamydia trachomatis; antigen; genital; vaccine; women

Comment in

PMID:
27738051
PMCID:
PMC5142095
DOI:
10.1093/infdis/jiw485
[Indexed for MEDLINE]
Free PMC Article

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