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J Renin Angiotensin Aldosterone Syst. 2016 Oct 12;17(4). pii: 1470320316669880. Print 2016 Oct.

Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint®.

Author information

1
Hypertension in Africa Research Team (HART), North-West University, Potchefstroom campus, South Africa johannes.vanrooyen@nwu.ac.za.
2
Attoquant Diagnostics, Austria.
3
Hypertension in Africa Research Team (HART), North-West University, Potchefstroom campus, South Africa.
4
Hypertension in Africa Research Team (HART), North-West University, Potchefstroom campus, South Africa MRC Extra Mural Unit: Hypertension and Cardiovascular Disease, North-West University, South Africa.

Abstract

OBJECTIVE:

The objective of this study was to make use of a quantitative and qualitative approach comparing the systemic renin-angiotensin system (RAS) of hypertensive black and white African men by using RAS equilibrium analysis.

MATERIALS AND METHODS:

This sub-study involved 23 black (n = 15) and white (n = 8) hypertensive men aged 39.5-41 years, living in the North West Province of South Africa. The RAS-Fingerprinting was determined with LC-MS/MS quantification of angiotensin peptides. Blood pressure and other variables were determined with known methods.

RESULTS:

The main finding of this study was the significant lower Ang I (<5.0 and 45.1 pg/ml; p = 0.005) and Ang II (15.6 and 123.9 pg/ml; p ⩽ 0.001) encountered in the hypertensive black African men compared to their white counterparts. Levels of Ang 1-5 (downstream metabolite of Ang 1-7) (1.8 and 3.0 pg/ml), were detected in black and white hypertensive men, respectively.

CONCLUSIONS:

The observed differences between circulating RAS components, which are reflected via equilibrium angiotensin levels, point to a distinctive molecular regulation of the RAAS in the two study cohorts. The increased peripheral resistance observed in hypertensive black individuals might take over a dominant role in control of blood pressure in this study population. A novel highly sensitive LC-MS/MS method resolved the issue of peptide recovery variations during sample preparation by using internal standards for each individual angiotensin metabolite.

KEYWORDS:

RAS peptides; RAS-Fingerprint; blacks; renin; whites

PMID:
27737932
PMCID:
PMC5843883
DOI:
10.1177/1470320316669880
[Indexed for MEDLINE]
Free PMC Article

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