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Parasit Vectors. 2016 Oct 13;9(1):547.

Population structure and dispersal routes of an invasive parasite, Fascioloides magna, in North America and Europe.

Author information

1
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia.
2
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia. hromadova@saske.sk.
3
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, Mlynská dolina, 842 15, Bratislava, Slovakia.
4
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia.
5
Geneton Ltd, Ilkovičova 3, 841 04, Bratislava, Slovakia.
6
Biology Centre CAS, Institute of Parasitology and Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic.
7
Department of Zoology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, Mlynská dolina, 842 15, Bratislava, Slovakia.
8
Alberta Fish and Wildlife Division and Department of Biological Sciences, University of Alberta, 6909-116 St, Edmonton, AB, T6H 4P2, Canada.

Abstract

BACKGROUND:

Fascioloides magna (Trematoda: Fasciolidae) is an important liver parasite of a wide range of free-living and domestic ruminants; it represents a remarkable species due to its large spatial distribution, invasive character, and potential to colonize new territories. The present study provides patterns of population genetic structure and admixture in F. magna across all enzootic regions in North America and natural foci in Europe, and infers migratory routes of the parasite on both continents.

METHODS:

In total, 432 individuals from five North American enzootic regions and three European foci were analysed by 11 microsatellite loci. Genetic data were evaluated by several statistical approaches: (i) the population genetic structure of F. magna was inferred using program STRUCTURE; (ii) the genetic interrelationships between populations were analysed by PRINCIPAL COORDINATES ANALYSIS; and (iii) historical dispersal routes in North America and recent invasion routes in Europe were explored using MIGRATE.

RESULTS:

The analysis of dispersal routes of the parasite in North America revealed west-east and south-north lineages that partially overlapped in the central part of the continent, where different host populations historically met. The exact origin of European populations of F. magna and their potential translocation routes were determined. Flukes from the first European focus, Italy, were related to F. magna from northern Pacific coast, while parasites from the Czech focus originated from south-eastern USA, particularly South Carolina. The Danube floodplain forests (third and still expanding focus) did not display relationship with any North American population; instead the Czech origin of the Danube population was indicated. A serial dilution of genetic diversity along the dispersion route across central and eastern Europe was observed. The results of microsatellite analyses were compared to previously acquired outputs from mitochondrial haplotype data and correlated with past human-directed translocations and natural migration of the final cervid hosts of F. magna.

CONCLUSIONS:

The present study revealed a complex picture of the population genetic structure and interrelationships of North American and European populations, global distribution and migratory routes of F. magna and an origin of European foci.

KEYWORDS:

Fascioloides magna; Genetic interrelationships; Giant liver fluke; Microsatellites; Migratory routes; Parasite

PMID:
27737705
PMCID:
PMC5064932
DOI:
10.1186/s13071-016-1811-z
[Indexed for MEDLINE]
Free PMC Article

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