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J Cell Physiol. 2017 Sep;232(9):2558-2568. doi: 10.1002/jcp.25646. Epub 2017 Apr 10.

Arabinogalactan Proteins From Baobab and Acacia Seeds Influence Innate Immunity of Human Keratinocytes In Vitro.

Author information

1
Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Végétal, Agronomie, Sol, et Innovation (VASI), GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.
2
BioEurope, Groupe SOLABIA, Anet, France.
3
Cell Imaging Platform (PRIMACEN-IRIB), Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
4
Laboratoire de Différenciation et Communication Neuronale et Neuroendocrine INSERM U982, IRIB, Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
5
Unite Mixte de Recherche 6270 CNRS-Laboratory "Polymères, Biopolymères, Surfaces", Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.

Abstract

Plant derived arabinogalactan proteins (AGP) were repeatedly confirmed as immunologically as well as dermatologically active compounds. However, little is currently known regarding their potential activity toward skin innate immunity. Here, we extracted and purified AGP from acacia (Acacia senegal) and baobab (Adansonia digitata) seeds to investigate their biological effects on the HaCaT keratinocyte cell line in an in vitro system. While AGP from both sources did not exhibit any cytotoxic effect, AGP from acacia seeds enhanced cell viability. Moreover, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that AGP extracted from both species induced a substantial overexpression of hBD-2, TLR-5, and IL1-α genes. These data suggest that plant AGP, already known to control plant defensive processes, could also modulate skin innate immune responses. J. Cell. Physiol. 232: 2558-2568, 2017.

PMID:
27736003
DOI:
10.1002/jcp.25646
[Indexed for MEDLINE]

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