Format

Send to

Choose Destination
Methods Mol Biol. 2017;1489:347-359.

Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches.

Author information

1
Center for Promotion of Dental Education and International Collaboration, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama City, Okayama, 700-8558, Japan.
2
Molecular Biology of Bones and Teeth Section, Craniofacial and Skeletal Diseases Branch, National Institutes of Health, Bethesda, MD, 20892, USA. myoung@dir.nidcr.nih.gov.
3
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama City, Okayama, 700-8558, Japan.

Abstract

Analysis of CCN4 function in bone was assessed using both gain and loss of function approaches. In mice this was done by genetic engineering and homologous recombination to create mice with complete ablation of the protein. For human skeletal cells adenoviral gene transfer and shRNA were used for gain and loss of function respectively. Here we describe procedures used to make and then analyze osteogenic and osteoclastic cells with or without CCN4 to determine its role in osteogenic differentiation.

KEYWORDS:

CCN4; Gene knockout; Osteogenic differentiation; Osteoprogenitor cell

PMID:
27734388
PMCID:
PMC6268190
DOI:
10.1007/978-1-4939-6430-7_29
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center