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Open Biol. 2016 Oct;6(10). pii: 160223.

The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition.

Author information

1
School of Life Sciences, University of Nottingham, Medical School, QMC, Nottingham NG7 2UH, UK.
2
School of Life Sciences, University of Nottingham, Medical School, QMC, Nottingham NG7 2UH, UK claire.friel@nottingham.ac.uk.

Abstract

Kinesins that influence the dynamics of microtubule growth and shrinkage require the ability to distinguish between the microtubule end and the microtubule lattice. The microtubule depolymerizing kinesin MCAK has been shown to specifically recognize the microtubule end. This ability is key to the action of MCAK in regulating microtubule dynamics. We show that the α4-helix of the motor domain is crucial to microtubule end recognition. Mutation of the residues K524, E525 and R528, which are located in the C-terminal half of the α4-helix, specifically disrupts the ability of MCAK to recognize the microtubule end. Mutation of these residues, which are conserved in the kinesin-13 family and discriminate members of this family from translocating kinesins, impairs the ability of MCAK to discriminate between the microtubule lattice and the microtubule end.

KEYWORDS:

ATP turnover; MCAK; depolymerization; kinesin-13; microtubule; microtubule end recognition

PMID:
27733589
PMCID:
PMC5090061
DOI:
10.1098/rsob.160223
[Indexed for MEDLINE]
Free PMC Article

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