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FASEB J. 2017 Jan;31(1):266-281. doi: 10.1096/fj.201600787RR. Epub 2016 Oct 12.

Pharmacologic activation of estrogen receptor β increases mitochondrial function, energy expenditure, and brown adipose tissue.

Author information

1
Department of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
2
Department of Preventive Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
3
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
4
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
5
Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
6
Molecular Informatics Core, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
7
Preclinical Research and Development, GTx, Incorporated, Memphis, Tennessee, USA.
8
Department of Comparative Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA; and.
9
Department of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA; rnaraya4@uthsc.edu.
10
West Cancer Center, Memphis, Tennessee, USA.

Abstract

Most satiety-inducing obesity therapeutics, despite modest efficacy, have safety concerns that underscore the need for effective peripherally acting drugs. An attractive therapeutic approach for obesity is to optimize/maximize energy expenditure by increasing energy-utilizing thermogenic brown adipose tissue. We used in vivo and in vitro models to determine the role of estrogen receptor β (ER-β) and its ligands on adipose biology. RNA sequencing and metabolomics were used to determine the mechanism of action of ER-β and its ligands. Estrogen receptor β (ER-β) and its selective ligand reprogrammed preadipocytes and precursor stem cells into brown adipose tissue and increased mitochondrial respiration. An ER-β-selective ligand increased markers of tricarboxylic acid-dependent and -independent energy biogenesis and oxygen consumption in mice without a concomitant increase in physical activity or food consumption, all culminating in significantly reduced weight gain and adiposity. The antiobesity effects of ER-β ligand were not observed in ER-β-knockout mice. Serum metabolite profiles of adult lean and juvenile mice were comparable, while that of adult obese mice was distinct, indicating a possible impact of obesity on age-dependent metabolism. This phenotype was partially reversed by ER-β-selective ligand. These data highlight a new role for ER-β in adipose biology and its potential to be a safer alternative peripheral therapeutic target for obesity.-Ponnusamy, S., Tran, Q. T., Harvey, I., Smallwood, H. S., Thiyagarajan, T., Banerjee, S., Johnson, D. L., Dalton, J. T., Sullivan, R. D., Miller, D. D., Bridges, D., Narayanan, R. Pharmacologic activation of estrogen receptor β increases mitochondrial function, energy expenditure, and brown adipose tissue.

KEYWORDS:

exercise mimetic; metabolic diseases; mitochondria; obesity; oxygen consumption

PMID:
27733447
PMCID:
PMC5161516
DOI:
10.1096/fj.201600787RR
[Indexed for MEDLINE]
Free PMC Article

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