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Nat Commun. 2016 Oct 12;7:13179. doi: 10.1038/ncomms13179.

NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis.

Author information

1
Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.
2
Arid Land Research Center, Tottori University, Tottori 680-0001, Japan.
3
PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan.
4
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.
5
Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada M5S3B2.
6
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G2W1.
7
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles, France.

Abstract

Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.

PMID:
27731416
PMCID:
PMC5064020
DOI:
10.1038/ncomms13179
[Indexed for MEDLINE]
Free PMC Article

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