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Nat Methods. 2016 Dec;13(12):1009-1011. doi: 10.1038/nmeth.4025. Epub 2016 Oct 10.

Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome.

Author information

1
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
2
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
3
Center for 3D Structure and Physics of the Genome, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Abstract

We present Micro-C XL, an improved method for analysis of chromosome folding at mononucleosome resolution. Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.

PMID:
27723753
DOI:
10.1038/nmeth.4025
[Indexed for MEDLINE]

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