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Virology. 2016 Dec;499:288-297. doi: 10.1016/j.virol.2016.09.023. Epub 2016 Oct 6.

A single intranasal immunization with a subunit vaccine formulation induces higher mucosal IgA production than live respiratory syncytial virus.

Author information

1
VIDO-InterVac, University of Saskatchewan, Saskatoon, Canada SK S7N 5E3.
2
Microbiology & Immunology, University of Saskatchewan, Saskatoon, Canada SK S7N 5E3.
3
VIDO-InterVac, University of Saskatchewan, Saskatoon, Canada SK S7N 5E3; Microbiology & Immunology, University of Saskatchewan, Saskatoon, Canada SK S7N 5E3.
4
VIDO-InterVac, University of Saskatchewan, Saskatoon, Canada SK S7N 5E3; Microbiology & Immunology, University of Saskatchewan, Saskatoon, Canada SK S7N 5E3. Electronic address: sylvia.vandenhurk@usask.ca.

Abstract

Respiratory syncytial virus (RSV) causes serious respiratory illness in infants and elderly. RSV infection induces short-lived immunity, which leaves people prone to re-infection. In contrast, the RSV fusion (F) protein formulated with a novel adjuvant (∆F/TriAdj) elicits long term protective immunity. A comparison of RSV-immunized mice to mice vaccinated with a single dose of ∆F/TriAdj showed no difference in IgG1 and IgG2a production; however, local IgA secreting memory B cell development and B cell IgA production were significantly lower in RSV vaccinated mice than in ∆F/TriAdj-immunized mice. This indicates a potential reason as to why long-term immunity is not induced by RSV infection. The comparison also revealed that germinal center lymphocyte populations were higher in ∆F/TriAdj-vaccinated mice. Furthermore, ∆F/TriAdj induced higher gene expression of activation-induced cytidine deaminase (AID), as well as IL-6, IL-21, TGF-β cytokines, which are key players in IgA class switch recombination, ultimately leading to a sustained long-term memory response.

KEYWORDS:

AID; Adjuvanted F protein; IgA; Immune memory; RSV

PMID:
27721128
DOI:
10.1016/j.virol.2016.09.023
[Indexed for MEDLINE]
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