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Elife. 2016 Oct 10;5. pii: e18383. doi: 10.7554/eLife.18383.

Asymmetric effects of activating and inactivating cortical interneurons.

Phillips EA1,2,3,4, Hasenstaub AR1,2,3,4.

Author information

1
Neuroscience Graduate Program, University of California, San Francisco, San Francisco, United States.
2
Center for Integrative Neuroscience, University of California San Francisco, San Francisco, United States.
3
Coleman Memorial Laboratory, University of California, San Francisco, San Francisco, United States.
4
Department of Otolaryngology-Head and Neck Surgery, University of California, San Francisco, San Francisco, United States.

Abstract

Bidirectional manipulations - activation and inactivation - are widely used to identify the functions supported by specific cortical interneuron types. Implicit in much of this work is the notion that tonic activation and inactivation will both produce valid, internally consistent insights into interneurons' computational roles. Here, using single-unit recordings in auditory cortex of awake mice, we show that this may not generally hold true. Optogenetically manipulating somatostatin-positive (Sst+) or parvalbumin-positive (Pvalb+) interneurons while recording tone-responses showed that Sst+ inactivation increased response gain, while Pvalb+ inactivation weakened tuning and decreased information transfer, implying that these neurons support delineable computational functions. But activating Sst+ and Pvalb+ interneurons revealed no such differences. We used a simple network model to understand this asymmetry, and showed how relatively small changes in key parameters, such as spontaneous activity or strength of the light manipulation, determined whether activation and inactivation would produce consistent or paradoxical conclusions regarding interneurons' computational functions.

KEYWORDS:

cortex; interneurons; mouse; neuroscience; optogenetics

PMID:
27719761
PMCID:
PMC5123863
DOI:
10.7554/eLife.18383
[Indexed for MEDLINE]
Free PMC Article

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