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Nanoscale. 2016 Oct 27;8(42):18087-18097.

Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration.

Author information

1
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. eunyang@kist.re.kr soyeonkim@kist.re.kr.
2
School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. mhyoon@gist.ac.kr.
3
Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon, 34141, Republic of Korea and Department of Functional Genomics, Korea University of Science and Technology (UST), KRIBB campus, Daejeon, 34141, Republic of Korea.
4
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. eunyang@kist.re.kr soyeonkim@kist.re.kr and Department of Biomedical Engineering, Korea University of Science and Technology (UST), KIST campus, Seoul 02792, Republic of Korea.
5
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. eunyang@kist.re.kr soyeonkim@kist.re.kr and Department of Biological Chemistry, Korea University of Science and Technology (UST), KIST campus, Seoul 02792, Republic of Korea.

Abstract

The biological applications of vertical nanostructures mostly rely on their intracellular accessibility through the cellular membrane by promoting cell-to-nanostructure interactions. Herein, we report a seemingly counter-intuitive approach for the spontaneous formation of mouse induced pluripotent stem cell (iPSC)-derived three-dimensional spherical colonies with unlimited self-renewal and differentiation potential. The comprehensive analyses of iPSCs cultured on vertical silicon nanocolumn arrays (vSNAs) with various nanocolumn geometries show reduced cell-to-substrate adhesion and enhanced cell-to-cell interactions under optimized vSNA conditions, successfully accommodating the spontaneous production of iPSC-derived spherical colonies. Remarkably, these colonies which were only minimally penetrated by and thereby easily harvested from wafer-sized vSNAs display a substantial increase in pluripotency marker expression and successfully differentiate into three germ layers. Our vSNAs capable of large-scale fabrication, efficient for spherical colony formation, and reusable for multiple iPSC culture could serve as a broad-impact culture platform for stem cell research.

PMID:
27714141
DOI:
10.1039/c6nr06203b
[Indexed for MEDLINE]

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