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Chem Soc Rev. 2016 Dec 21;45(24):6666-6684. Epub 2016 Sep 14.

CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering.

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CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi 110025, India.


The CRISPR-Cas9 system has revolutionized the process of making changes to the DNA sequence of organisms. Relying on a simplistic model of RNA guided DNA binding and cleavage, this molecular toolbox has found application in nearly every branch of biological sciences. The story of CRISPR-Cas9 is one of discovery and development where a component of bacterial adaptive immunity has been harnessed to address important biological questions using significant inputs from physicochemical structure-function studies. In this review, we trace the evolution of CRISPR-Cas9 from its predecessor genome editing tools and document its current status with an emphasis on chemical biology aspects of modulating its activity to generate a potent tool for gene therapy applications.

[Indexed for MEDLINE]

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