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Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):E6476-E6485. Epub 2016 Oct 5.

The Architecture of Trypanosoma brucei editosomes.

Author information

1
Center for Infectious Disease Research, Seattle, WA 98109.
2
Institute for Systems Biology, Seattle, WA 98109.
3
Institute for Systems Biology, Seattle, WA 98109 jranish@systemsbiology.org ken.stuart@cidresearch.org.
4
Center for Infectious Disease Research, Seattle, WA 98109; jranish@systemsbiology.org ken.stuart@cidresearch.org.

Abstract

Uridine insertion and deletion RNA editing generates functional mitochondrial mRNAs in Trypanosoma brucei Editing is catalyzed by three distinct ∼20S editosomes that have a common set of 12 proteins, but are typified by mutually exclusive RNase III endonucleases with distinct cleavage specificities and unique partner proteins. Previous studies identified a network of protein-protein interactions among a subset of common editosome proteins, but interactions among the endonucleases and their partner proteins, and their interactions with common subunits were not identified. Here, chemical cross-linking and mass spectrometry, comparative structural modeling, and genetic and biochemical analyses were used to define the molecular architecture and subunit organization of purified editosomes. We identified intra- and interprotein cross-links for all editosome subunits that are fully consistent with editosome protein structures and previously identified interactions, which we validated by genetic and biochemical studies. The results were used to create a highly detailed map of editosome protein domain proximities, leading to identification of molecular interactions between subunits, insights into the functions of noncatalytic editosome proteins, and a global understanding of editosome architecture.

KEYWORDS:

RNA editing; Trypanosoma brucei; cross-linking; editosome; proteomics

PMID:
27708162
PMCID:
PMC5081628
DOI:
10.1073/pnas.1610177113
[Indexed for MEDLINE]
Free PMC Article

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