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J Exp Bot. 2016 Nov;67(21):6125-6138. Epub 2016 Oct 5.

Robust phenotyping strategies for evaluation of stem non-structural carbohydrates (NSC) in rice.

Author information

  • 1Section of Plant Breeding and Genetics, School of Integrated Plant Sciences, Cornell University, Ithaca, NY 14853-1901, USA.
  • 2Integrated Biorefinery Research Facility, National Renewable Energy Lab, Golden, CO 80401, USA.
  • 3International Center for Tropical Agriculture, Km17 Recta Cali-Palmira, PO Box 6713, Cali, Colombia.
  • 4International Rice Research Institute, Los Baños, Laguna, Philippines.
  • 5Bayesic Research, Ithaca, NY 14850, USA.
  • 6Section of Plant Breeding and Genetics, School of Integrated Plant Sciences, Cornell University, Ithaca, NY 14853-1901, USA srm4@cornell.edu.

Abstract

Rice plants (Oryza sativa) accumulate excess photoassimilates in the form of non-structural carbohydrates (NSCs) in their stems prior to heading that can later be mobilized to supplement photosynthate production during grain-filling. Despite longstanding interest in stem NSC for rice improvement, the dynamics of NSC accumulation, remobilization, and re-accumulation that have genetic potential for optimization have not been systematically investigated. Here we conducted three pilot experiments to lay the groundwork for large-scale diversity studies on rice stem NSC. We assessed the relationship of stem NSC components with 21 agronomic traits in large-scale, tropical yield trials using 33 breeder-nominated lines, established an appropriate experimental design for future genetic studies using a Bayesian framework to sample sub-datasets from highly replicated greenhouse data using 36 genetically diverse genotypes, and used 434 phenotypically divergent rice stem samples to develop two partial least-squares (PLS) models using near-infrared (NIR) spectra for accurate, rapid prediction of rice stem starch, sucrose, and total non-structural carbohydrates. We find evidence that stem reserves are most critical for short-duration varieties and suggest that pre-heading stem NSC is worthy of further experimentation for breeding early maturing rice.

KEYWORDS:

Oryza sativa ; Near-infrared spectroscopy; non-structural carbohydrates; partial least-squares; replication; yield potential.

PMID:
27707775
PMCID:
PMC5100024
DOI:
10.1093/jxb/erw375
[PubMed - in process]
Free PMC Article
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