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Mol Gen Genet. 1989 Jun;217(2-3):324-31.

Identification and characterization of a locus inhibiting extrachromosomal maintenance of the Streptomyces plasmid SLP1.

Author information

1
Department of Genetics, Stanford University School of Medicine, CA 94305.

Abstract

We report here the existence and initial characterization of a genetic locus (imp) that inhibits maintenance of SLP1-derived plasmids as extrachromosomal replicons in a manner distinct from normal incompatibility between autonomous SLP1 replicons. The trans-acting imp function has been localized to a 1.8 kb Eco47III restriction fragment present on integrated SLP1 elements. At least part of this DNA segment is absent from SLP1-derived plasmids. DNA sequence analysis of the imp region indicates that it contains three overlapping open reading frames (ORFs) that may constitute a polycistronic operon. The effects of insertions within the imp region indicate that uninterrupted transcription through all three ORFs is necessary for imp activity.

PMID:
2770697
DOI:
10.1007/bf02464900
[Indexed for MEDLINE]

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