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Behav Genet. 2017 Mar;47(2):227-243. doi: 10.1007/s10519-016-9819-x. Epub 2016 Oct 5.

Genetic and Genomic Response to Selection for Food Consumption in Drosophila melanogaster.

Garlapow ME1,2,3, Everett LJ1,2,3,4, Zhou S1,2,3,4, Gearhart AW2,3, Fay KA2,3, Huang W1,2,3,4, Morozova TV2,3, Arya GH2,3, Turlapati L2,3, St Armour G2,3, Hussain YN2,3, McAdams SE2,3, Fochler S2,3,5, Mackay TF6,7,8,9.

Author information

1
Program in Genetics, North Carolina State University, Raleigh, NC, 27695-7614, USA.
2
Department of Biological Sciences, North Carolina State University, Raleigh, NC, 27695-7614, USA.
3
W. M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC, 27695-7614, USA.
4
Initiative for Biological Complexity, North Carolina State University, Raleigh, NC, 27695-7614, USA.
5
School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.
6
Program in Genetics, North Carolina State University, Raleigh, NC, 27695-7614, USA. trudy_mackay@ncsu.edu.
7
Department of Biological Sciences, North Carolina State University, Raleigh, NC, 27695-7614, USA. trudy_mackay@ncsu.edu.
8
W. M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC, 27695-7614, USA. trudy_mackay@ncsu.edu.
9
Initiative for Biological Complexity, North Carolina State University, Raleigh, NC, 27695-7614, USA. trudy_mackay@ncsu.edu.

Abstract

Food consumption is an essential component of animal fitness; however, excessive food intake in humans increases risk for many diseases. The roles of neuroendocrine feedback loops, food sensing modalities, and physiological state in regulating food intake are well understood, but not the genetic basis underlying variation in food consumption. Here, we applied ten generations of artificial selection for high and low food consumption in replicate populations of Drosophila melanogaster. The phenotypic response to selection was highly asymmetric, with significant responses only for increased food consumption and minimal correlated responses in body mass and composition. We assessed the molecular correlates of selection responses by DNA and RNA sequencing of the selection lines. The high and low selection lines had variants with significantly divergent allele frequencies within or near 2081 genes and 3526 differentially expressed genes in one or both sexes. A total of 519 genes were both genetically divergent and differentially expressed between the divergent selection lines. We performed functional analyses of the effects of RNAi suppression of gene expression and induced mutations for 27 of these candidate genes that have human orthologs and the strongest statistical support, and confirmed that 25 (93 %) affected the mean and/or variance of food consumption.

KEYWORDS:

CAFE assay; DNA-seq; Feeding behavior; RNA-seq; Realized heritability

PMID:
27704301
PMCID:
PMC5305434
DOI:
10.1007/s10519-016-9819-x
[Indexed for MEDLINE]
Free PMC Article

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